DNA Extraction Columns Contaminated with Murine Sequences

DNA Extraction Columns Contaminated with Murine Sequences

Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue sampl...

Mitochondrial DNA and filtering of contaminated sequences

We analyzed a total of 27,958,007 sequences that all had perfect matches to the expected indices (Extended Data Table 6). We merged the reads from either end of the same molecule by requiring an overlap of at least 11 base pairs, and generated a consensus sequence by picking the base with the higher sequence quality. We mapped these merged reads, which we call a “fragment”, to the revised Cambr...

Troubleshooting Liquid-liquid Extraction Columns

Process engineers can be faced with the task of understanding and optimizing the performance of an existing liquid-liquid extraction (extraction) column. Often times, an extraction column has been operating many years, and the performance is not well understood by the personnel responsible for its operation. Since this technology is not treated comprehensively during most under-graduate Chemica...

PCR Master Mixes Harbour Murine DNA Sequences. Caveat Emptor!

BACKGROUND XMRV is the most recently described retrovirus to be found in Man, firstly in patients with prostate cancer (PC) and secondly in 67% of patients with chronic fatigue syndrome (CFS) and 3.7% of controls. Both disease associations remain contentious. Indeed, a recent publication has concluded that "XMRV is unlikely to be a human pathogen". Subsequently related but different polytropic ...

Prediction of Mean Drop Size in Pulsed Packed Extraction Columns